MP08-08 NERVE TRANSFER FOR RESTORATION OF LOWER MOTOR NEURON-LESIONED BLADDER FUNCTION. PART 2: ATTENUATION OF PURINERGIC BLADDER SMOOTH MUSCLE CONTRACTIONS

You have accessJournal of UrologyBladder & Urethra: Anatomy, Physiology Pharmacology (MP08)1 Sep 2021MP08-08 NERVE TRANSFER FOR RESTORATION OF LOWER MOTOR NEURON-LESIONED BLADDER FUNCTION. PART 2: ATTENUATION PURINERGIC SMOOTH MUSCLE CONTRACTIONS Nagat Frara, Dania Giaddui, Alan S. Braverman, Danielle Porecca, Justin M. Brown, Michael Mazzei, Ida J. Wagner, Michel A. Pontari, Ekta Tiwari, Courtney L. Testa, Daohai Yu, Locas Hobson, Mary F. Barbe, and R. Ruggieri FraraNagat Frara More articles by this author , GiadduiDania Giaddui BravermanAlan Braverman PoreccaDanielle Porecca BrownJustin Brown MazzeiMichael Mazzei WagnerIda Wagner PontariMichel Pontari TiwariEkta Tiwari TestaCourtney Testa YuDaohai Yu HobsonLocas Hobson BarbeMary Barbe RuggieriMichael View All Author Informationhttps://doi.org/10.1097/JU.0000000000001981.08AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: This study determined the effect pelvic organ long-term decentralization reinnervation one year later on contribution muscarinic purinergic receptors ex-vivo, nerve-evoked, bladder smooth muscle contractions. METHODS: Nineteen canines underwent bilateral transection all coccygeal sacral (S) spinal roots, dorsal roots lumbar (L)7 hypogastric nerves. After exclusions, 8 were reinnervated 12 months post-decentralization with obturator-to-pelvic sciatic-to-pudendal nerve transfers then euthanized 8-12 later; four served as decentralized only animals. Controls included six sham-operated three unoperated Bladder tissues assessed for contractile responses potassium chloride (KCl) electric field stimulation (EFS) before after receptor desensitization alpha, beta-methylene adenosine triphosphate (α,β-mATP), antagonism atropine, or sodium channel blockade tetrodotoxin. RESULTS: Atropine inhibition EFS-induced contractions increased 26%in 34%in animals compared controls. Maximal α,β-mATP did not differ between groups. In strips from animals, response EFS was enhanced 52% at lower frequencies KCl normal CONCLUSIONS: The observation nerve-evoked antagonist no change in responsiveness agonist suggests either decreased ATP release ectoATPase activity detrusor a consequence decentralization. reduction frequency required produce maximum contraction following may be due sensitivity effectiveness neurotransmission. Source Funding: NINDS R01NS070267 © 2021 American Urological Association Education Research, Inc.FiguresReferencesRelatedDetails Volume 206Issue Supplement 3September 2021Page: e153-e153 Advertisement Copyright Permissions© Inc.MetricsAuthor Information Expand Loading ...